Real-time PCR in virology

IM Mackay, KE Arden, A Nitsche - Nucleic acids research, 2002 - academic.oup.com
The use of the polymerase chain reaction (PCR) in molecular diagnostics has increased to
the point where it is now accepted as the gold standard for detecting nucleic acids from a …

[PDF][PDF] SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically proven protease inhibitor

M Hoffmann, H Kleine-Weber, S Schroeder, N Krüger… - cell, 2020 - cell.com
The recent emergence of the novel, pathogenic SARS-coronavirus 2 (SARS-CoV-2) in
China and its rapid national and international spread pose a global health emergency. Cell …

[HTML][HTML] Olfactory transmucosal SARS-CoV-2 invasion as a port of central nervous system entry in individuals with COVID-19

J Meinhardt, J Radke, C Dittmayer, J Franz… - Nature …, 2021 - nature.com
The newly identified severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
causes COVID-19, a pandemic respiratory disease. Moreover, thromboembolic events …

Guideline to reference gene selection for quantitative real-time PCR

A Radonić, S Thulke, IM Mackay, O Landt… - Biochemical and …, 2004 - Elsevier
Today, quantitative real-time PCR is the method of choice for rapid and reliable
quantification of mRNA transcription. However, for an exact comparison of mRNA …

[HTML][HTML] Monkeypox (Mpox) requires continued surveillance, vaccines, therapeutics and mitigating strategies

RL Roper, A Garzino-Demo, C Del Rio, C Bréchot… - Vaccine, 2023 - Elsevier
The widespread outbreak of the monkeypox virus (MPXV) recognized in 2022 poses new
challenges for public healthcare systems worldwide. With more than 86,000 people infected …

Investigating the zoonotic origin of the West African Ebola epidemic

A Marí Saéz, S Weiss, K Nowak, V Lapeyre… - EMBO molecular …, 2015 - embopress.org
The severe Ebola virus disease epidemic occurring in West Africa stems from a single
zoonotic transmission event to a 2‐year‐old boy in Meliandou, Guinea. We investigated the …

[PDF][PDF] Pandemic human viruses cause decline of endangered great apes

S Köndgen, H Kühl, PK N'Goran, PD Walsh, S Schenk… - Current Biology, 2008 - cell.com
Commercial hunting and habitat loss are major drivers of the rapid decline of great apes [1].
Ecotourism and research have been widely promoted as a means of providing alternative …

[HTML][HTML] Temporal and spatial analysis of the 2014–2015 Ebola virus outbreak in West Africa

MW Carroll, DA Matthews, JA Hiscox, MJ Elmore… - Nature, 2015 - nature.com
West Africa is currently witnessing the most extensive Ebola virus (EBOV) outbreak so far
recorded,,. Until now, there have been 27,013 reported cases and 11,134 deaths. The origin …

[HTML][HTML] Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections

VM Corman, MA Müller, U Costabel, J Timm… - …, 2012 - eurosurveillance.org
We present a rigorously validated and highly sensitive confirmatory real-time RT-PCR assay
(1A assay) that can be used in combination with the previously reported upE assay. Two …

[HTML][HTML] Equination (inoculation of horsepox): An early alternative to vaccination (inoculation of cowpox) and the potential role of horsepox virus in the origin of the …

J Esparza, L Schrick, CR Damaso, A Nitsche - Vaccine, 2017 - Elsevier
For almost 150 years after Edward Jenner had published the “Inquiry” in 1798, it was
generally assumed that the cowpox virus was the vaccine against smallpox. It was not until …