Resurgence of Ebola Virus Disease in Guinea Linked to a Survivor With Virus Persistence in Seminal Fluid for More Than 500 Days

Boubacar Diallo; Daouda Sissoko; Nicholas J Loman; Hadja Aïssatou Bah; Hawa Bah; Mary Claire Worrell; Lya Saidou Conde; Ramata Sacko; Samuel Mesfin; Angelo Loua; Jacques Katomba Kalonda; Ngozi A Erondu; Benjamin A Dahl; Susann Handrick; Ian Goodfellow; Luke W Meredith; Matthew Cotten; Umaru Jah; Raoul Emeric Guetiya Wadoum; Pierre Rollin; N'Faly Magassouba; Denis Malvy; Xavier Anglaret; Miles W Carroll; Raymond Bruce Aylward; Mamoudou Harouna Djingarey; Abdoulaye Diarra; Pierre Formenty; Sakoba Keïta; Stephan Günther; Andrew Rambaut; Sophie Duraffour

doi:10.1093/cid/ciw601

Resurgence of Ebola Virus Disease in Guinea Linked to a Survivor With Virus Persistence in Seminal Fluid for More Than 500 Days

Clin Infect Dis. 2016 Nov 15;63(10):1353-1356. doi: 10.1093/cid/ciw601. Epub 2016 Sep 1.

Authors

Boubacar Diallo¹, Daouda Sissoko^{2

3}, Nicholas J Loman⁴, Hadja Aïssatou Bah⁵, Hawa Bah¹, Mary Claire Worrell^{6

7}, Lya Saidou Conde¹, Ramata Sacko¹, Samuel Mesfin¹, Angelo Loua¹, Jacques Katomba Kalonda⁶, Ngozi A Erondu⁶, Benjamin A Dahl^{6

7}, Susann Handrick^{8

9}, Ian Goodfellow^{10

11}, Luke W Meredith^{10

11}, Matthew Cotten¹², Umaru Jah¹¹, Raoul Emeric Guetiya Wadoum^{11

13}, Pierre Rollin^{6

14}, N'Faly Magassouba¹⁵, Denis Malvy^{2

3}, Xavier Anglaret^{2

16}, Miles W Carroll^{8

17

18}, Raymond Bruce Aylward¹⁹, Mamoudou Harouna Djingarey¹, Abdoulaye Diarra¹, Pierre Formenty¹⁹, Sakoba Keïta²⁰, Stephan Günther^{8

9}, Andrew Rambaut²¹, Sophie Duraffour^{8

9}

Affiliations

¹ World Health Organization, Conakry, Guinea.
² INSERM U1219, Bordeaux University.
³ Bordeaux University Hospital, France.
⁴ Institute of Microbiology and Infection, University of Birmingham, United Kingdom.
⁵ Laboratoire des Fièvres Hémorragiques en Guinée, Hôpital Donka et N'Zérékoré.
⁶ Centers for Disease Control and Prevention (CDC) Guinea Response Team, Conakry.
⁷ Center for Global Health, CDC, Atlanta, Georgia.
⁸ European Mobile Laboratory Consortium.
⁹ Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
¹⁰ Division of Virology, Department of Pathology, University of Cambridge, Addenbrooke's Hospital, United Kingdom.
¹¹ Department of Public Health, University of Makeni, Sierra Leone.
¹² Wellcome Genome Campus, Hinxton, Cambridge, United Kingdom.
¹³ Department of Biology, University of Rome II "Tor Vergata," Italy.
¹⁴ National Center for Emerging and Zoonotic Infectious Diseases, CDC, Atlanta, Georgia.
¹⁵ Université Gamal Abdel Nasser de Conakry, Laboratoire des Fièvres Hémorragiques en Guinée, Guinea.
¹⁶ Programme ANRS Coopération Côte d'Ivoire, Agence Nationale de Recherche sur le Sida Research Site, Treichville University Hospital, Abidjan, Côte d'Ivoire.
¹⁷ Public Health England, Porton Down, Salisbury.
¹⁸ University of Southampton, South General Hospital, United Kingdom.
¹⁹ World Health Organization, Geneva, Switzerland.
²⁰ Ministry of Health, Conakry, Guinea.
²¹ Institute for Evolutionary Biology, Centre for Infection, Immunity and Evolution, Ashworth Laboratories, University of Edinburgh, United Kingdom.

Abstract

We report on an Ebola virus disease (EVD) survivor who showed Ebola virus in seminal fluid 531 days after onset of disease. The persisting virus was sexually transmitted in February 2016, about 470 days after onset of symptoms, and caused a new cluster of EVD in Guinea and Liberia.

Keywords: Ebola virus; Ebola virus persistence; real-time sequencing; seminal fluid; sexual transmission.

MeSH terms

Disease Outbreaks*
Ebolavirus / genetics*
Ebolavirus / isolation & purification
Female
Guinea
Hemorrhagic Fever, Ebola* / transmission
Hemorrhagic Fever, Ebola* / virology
Humans
Male
Polymerase Chain Reaction
RNA, Viral / analysis
Semen / virology*
Sexually Transmitted Diseases, Viral* / transmission
Sexually Transmitted Diseases, Viral* / virology
Survivors

Substances

RNA, Viral

Abstract

MeSH terms

Substances

Grants and funding