Molecular diagnostics of viral hemorrhagic fevers
Section snippets
Laboratory diagnosis of pathogens causing syndromes similar to VHF
Important pathogens which can cause symptoms similar to VHF are Plasmodium falciparum, Neisseria meningitidis, Leptospira interrogans, and viruses causing fulminant hepatitis. Hence, a close collaboration between laboratories specialized in VHF diagnostics and facilities able to diagnose these pathogens is desirable.
Especially malaria tropica can resemble a VHF and should always be excluded in travelers with fever who are returning from endemic areas, even if prophylaxis was taken. Malaria is
Acknowledgements
The work of the authors is supported by grants from the Bundesministerium für Gesundheit (Grant 325-4539-85/3) and from the Bundesamt für Wehrtechnik und Beschaffung (grants E/B31E/M0171/M5916 and E/B41G/1G309/1A403). The Bernhard-Nocht-Institute is supported by the Bundesministerium für Gesundheit and the Freie und Hansestadt Hamburg. We thank W. Preiser (University of Frankfurt/Main), M. Niedrig, and W. Haas (both Robert-Koch-Institute Berlin) for communicating unpublished results.
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Development of multivalent vaccine targeting M segment of Crimean Congo Hemorrhagic Fever Virus (CCHFV) using immunoinformatic approaches
2022, Saudi Journal of Biological SciencesCitation Excerpt :Molecular as well as serological assays such as nucleic acid tests (NAT) for RNA detection, IgG (for survivors), IgM (acute viral infection) and antigen (Ag) capture ELISA, are typically used to detect CCHF in human and animals (World Health Organization, n.d.; Mazzola and Kelly-Cirino, 2019). However, there are certain limitations, such as viral genetic diversity (World Health Organization, n.d.) and lack of a detectable antibody response in hosts (Drosten et al., 2003; Fernandez-García et al., 2014). Consequently, at present, there is no universally accepted gold standard (reference test) for CCHFV (World Health Organization, n.d.).
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2019, Infectious Disease Clinics of North AmericaDevelopment of an RT-LAMP assay for the detection of Lassa viruses in southeast and south-central Nigeria
2019, Journal of Virological MethodsCitation Excerpt :Presently, LASV diagnostics are based on the detection of viral antigens, nucleic acids, and IgM antibodies. To identify LASV, enzyme-linked immunosorbent assay (ELISA), reverse transcription polymerase chain reaction (RT-PCR), and tissue culture methods have been developed (Drosten et al., 2003; Hallam et al., 2018; Hamblion et al., 2018). However, owing to their high sensitivity and specificity, conventional and real-time RT-PCR are the chosen reference methods for the diagnosis of LF (Raabe and Koehler, 2017).
Essentials of filoviral load quantification
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