Table 3

HSV-2 diagnostics research progress according to the associated research priorities set in the 2001 WHO HSV-2 workshop

WHO 2001 research priority: HSV-2 diagnosticsHas the priority been addressed?Explanation
YesPartiallyNO
Optimal sampling techniques for direct diagnostic methods Embedded Image
  • Self-collection validated in two studies, more studies required for enhanced repeatability.

  • Cervicovaginal lavage superior to endocervicovaginal swabbing, one study.

  • Cervicovaginal lavage enhanced with swabbing an accurate method, one study.

Quantification techniques for HSV-2 DNA detection Embedded Image Embedded Image Embedded Image Embedded Image Embedded Image
  • Several techniques studies

  • Repeatability between studies

  • DNA detection methods have become the preferred direct technique over the 20-year time period.

Development of a rapid diagnostic test for genital herpes diagnosis Embedded Image Embedded Image Embedded Image Embedded Image Embedded Image Embedded Image Embedded Image Embedded Image Embedded Image
  • Insufficient accuracy of all assays

  • Only two assays evaluated (Biokit and Herpeselect Rapid)

  • No development of new assay

Performance of existing serological tests in different African sera Embedded Image Embedded Image Embedded Image Embedded Image Embedded Image
  • ELlSA assays tested in several African regions/populations.

  • Best performing assay determined

  • Effect of HIV infection on assay performance evaluated.

  • Optimal assay cut-off index values determined

  • HSV-2, herpes simplex virus type 2.