Test type | Reference | Review/EQA | Labs using the method (n)65 | CCHFV target54 |
qRT-PCR | 73 | 54 65 | 3 | 24 genomic targets |
qRT-PCR | 113 | 54 | – | 19 strains worldwide |
qRT-PCR | 76 78 | 53 54 59 65 | 2 | Kosovo Hoti and Drosdov strains |
qRT-PCR | 114, updated 57 | 54 | – | 18 strains worldwide (updated 26) |
qRT-PCR | 115 116 | 65 | 3 | – |
qRT-PCR | 117 118 | 53 54 59 65 | 12 | 17 strains worldwide |
qRT-PCR | 119 | 53 59 65 | 2 | – |
qRT-PCR | 120 | 54 | – | 19 Southern African strains |
qRT-PCR | 121 | 54 | – | All known worldwide strains, including the AP92 strain |
qRT-PCR | 122 | – | – | – |
Nested RT-PCR | 123 | 53 59 65 | 2 | – |
Nested RT-PCR | 74 | 53 65 | 2 | – |
Nested RT-PCR | 22 | 65 | 1 | – |
Nested RT-PCR | 124 | – | – | – |
Conventional RT-PCR | 72 125 | 54 65 | 2 | 7 geographically diverse strains |
Isothermal RPA | 82 | – | – | 7 geographically diverse strains |
PCR multiplex | 64 92 | – | 2 | – |
Low density array | 118 | 53 54 | – | Strains worldwide |
High density array | 90 | 54 | – | Nigerian strain |
Multiplex RT-PCR/NGS | 84 | – | – | 46 VHF species |
CCHF, Crimean-Congo haemorrhagic fever; CCHFV, CCHF virus; EQA, external quality assessment; NAAT, nucleic acid amplification test; NGS, next-generation sequencing; qRT-PCR, quantitative real-time RT-PCR; RPA, recombinase polymerase amplification; VHF, viral haemorrhagic fever.