Table 2

Inhouse NAAT tests for CCHF: references, reviews, EQA

Test typeReferenceReview/EQALabs using the method (n)65 CCHFV target54
qRT-PCR 73 54 65 324 genomic targets
qRT-PCR 113 54 19 strains worldwide
qRT-PCR 76 78 53 54 59 65 2Kosovo Hoti and Drosdov strains
qRT-PCR 114, updated 57 54 18 strains worldwide (updated 26)
qRT-PCR 115 116 65 3
qRT-PCR 117 118 53 54 59 65 1217 strains worldwide
qRT-PCR 119 53 59 65 2
qRT-PCR 120 54 19 Southern African strains
qRT-PCR 121 54 All known worldwide strains, including the AP92 strain
qRT-PCR 122
Nested RT-PCR 123 53 59 65 2
Nested RT-PCR 74 53 65 2
Nested RT-PCR 22 65 1
Nested RT-PCR 124
Conventional RT-PCR 72 125 54 65 27 geographically diverse strains
Isothermal RPA 82 7 geographically diverse strains
PCR multiplex 64 92 2
Low density array 118 53 54 Strains worldwide
High density array 90 54 Nigerian strain
Multiplex RT-PCR/NGS 84 46 VHF species
  • CCHF, Crimean-Congo haemorrhagic fever; CCHFV, CCHF virus; EQA, external quality assessment; NAAT, nucleic acid amplification test; NGS, next-generation sequencing; qRT-PCR, quantitative real-time RT-PCR; RPA, recombinase polymerase amplification; VHF, viral haemorrhagic fever.