Assay type | Target | Multiplex | Detection | Detection limit | Reference |
RT-PCR | S segment (GPC) | – | Acrylamide gel | 102 TCID50 | 110 |
RT-PCR | S segment (GPC) | – | Agarose gel/ Southern blot | 101.6 TCID50 | 118 |
RT-PCR | S segment (GPC) | – | Standard | 1–10 copies/assay | 45 109 144 |
real-time RT-PCR (qRT-PCR) | S segment (GPC) | – | SYBR-green | 8.6–16 copies/assay 1545–2835 copies/mL | 45 144 152 |
RT-PCR | S segment (NP) | 10-plex | Mass spectrometry | 20 copies/assay | 138 |
RT-PCR | S segment (NP) | >20-plex | Microarray | 1900 copies/assay | 139 |
RT-PCR | L segment (LP) | – | Standard | 4290 copies/mL | 144 153 |
RT-PCR | L segment (LP) | 8-plex | Standard | 1200 copies/assay 105 copies/mL | 137 |
RT-PCR | S segment (GPC) | – | Standard | 4–30 copies/assay 342–2560 copies/mL | 45 107 144 |
qRT-PCR | S segment (GPC), L segment (LP), | – | SYBR-green | n/a | 45 123 |
One-step qRT-PCR | S segment (GPC, NP) | – | Standard | 234–583 copies | 45 141 |
RT-PCR | S segment (GPC), L segment (LP) | – | Standard | 4290 copies/mL (serial assays) | 122 144 |
RT-LAMP | S segment (GPC) | – | Turbidity | 100 copies | 154 |
One-step RT-PCR | S segment (GPC) | – | Microarray | 1540 copies/mL | 45 155 |
One-step qRT-PCR | S segment (GPC) | 4-plex | Standard | 45–150 copies/assay | 136 |
One-step RT-PCR/LDR | L segment (LP) | 11-plex | Microarray | 100 copies/mL | 140 |
LAMP, loop-mediated isothermal amplification; LASV, Lassa fever virus; LDR, ligase detection reaction; NAAT, nucleic acid amplification test; RT, reverse transcriptase; q, quantitative.