Table 2

In-house NAAT tests for LASV

Assay typeTargetMultiplexDetectionDetection limitReference
RT-PCRS segment (GPC)Acrylamide gel102 TCID50 110
RT-PCRS segment (GPC)Agarose gel/ Southern blot101.6 TCID50 118
RT-PCRS segment (GPC)Standard1–10 copies/assay 45 109 144
real-time RT-PCR
(qRT-PCR)
S segment (GPC)SYBR-green8.6–16 copies/assay 1545–2835 copies/mL 45 144 152
RT-PCRS segment (NP)10-plexMass spectrometry20 copies/assay 138
RT-PCRS segment (NP)>20-plexMicroarray1900 copies/assay 139
RT-PCRL segment (LP)Standard4290 copies/mL 144 153
RT-PCRL segment (LP)8-plexStandard1200 copies/assay 105 copies/mL 137
RT-PCRS segment (GPC)Standard4–30 copies/assay 342–2560 copies/mL 45 107 144
qRT-PCRS segment (GPC), L segment (LP),SYBR-greenn/a 45 123
One-step qRT-PCRS segment (GPC, NP)Standard234–583 copies 45 141
RT-PCRS segment (GPC), L segment (LP)Standard4290 copies/mL (serial assays) 122 144
RT-LAMPS segment (GPC)Turbidity100 copies 154
One-step RT-PCRS segment (GPC)Microarray1540 copies/mL 45 155
One-step qRT-PCRS segment (GPC)4-plexStandard45–150 copies/assay 136
One-step RT-PCR/LDRL segment (LP)11-plexMicroarray100 copies/mL 140
  • LAMP, loop-mediated isothermal amplification; LASV, Lassa fever virus; LDR, ligase detection reaction; NAAT, nucleic acid amplification test; RT, reverse transcriptase; q, quantitative.