Table 3

In-house NAAT tests for NiV*

Assay typeTargetReference laboratoryReference
RT-PCRNiV P, N genesICDDRB and IEDCR,
CDC (USA)
7 45
Real-time RT-PCRNiV N geneInstitut Pasteur (France) 112
SYBR qRT-PCRNiV N geneUniversity of Malaya (Malaysia) 113
Duplex nested RT-PCRNiV N geneChulalongkorn University Hospital (Thailand) 114
Nested RT-PCRHeV/NiV P geneBernhard Nocht Institute (Germany), CDC (USA) 18 115
Nested RT-PCRNiV L geneCSIRO/AAHL, University of Cambridge, Zoological Society (UK) 71
SYBR qRT-PCRNiV N geneCSIRO/AAHL, University of Cambridge, Zoological Society (UK) 71
Real-time RT-PCRNiV/HeV P, M, N genesCSIRO/AAHL, University of Cambridge, Zoological Society (UK) 71
Multiplex bead-based real-time RT-PCRNiV, HeV
N and P genes
CSIRO/AAHL (Australia) 89
Multiplex array card real-time RT-PCR26 multiplex panel including NiV N geneCDC (Kenya, USA) 90
Multiplex array card real-time RT-PCR21 multiplex panel including NiV N geneKenya Medical Research Institute (Kenya), CDC (Kenya, USA) 91
  • *Adapted with permission from Springer Customer Service Centre GmBH: Springer Nature Indian Journal of Virology,66 copyright 2013; Springer Customer Service Centre GmBH: Springer Nature Springer eBook,67 copyright 2012; and World Organisation for Animal Health (OIE).70

  • AAHL, Australian Animal Health Laboratory; CDC, Centers for Disease Control and Prevention; CSIRO, Commonwealth Scientific and Industrial Research Organisation; HeV, Hendra virus; ICDDRB, International Centre for Diarrhoeal Disease Research, Bangladesh; IEDCR, Institute of Epidemiology, Disease Control and Research; NiV, Nipah virus; RT-PCR, reverse transcriptase PCR; qRT-PCR, quantitative RT-PCR.