Assay type | Target | Reference laboratory | Reference |
RT-PCR | NiV P, N genes | ICDDRB and IEDCR, CDC (USA) | 7 45 |
Real-time RT-PCR | NiV N gene | Institut Pasteur (France) | 112 |
SYBR qRT-PCR | NiV N gene | University of Malaya (Malaysia) | 113 |
Duplex nested RT-PCR | NiV N gene | Chulalongkorn University Hospital (Thailand) | 114 |
Nested RT-PCR | HeV/NiV P gene | Bernhard Nocht Institute (Germany), CDC (USA) | 18 115 |
Nested RT-PCR | NiV L gene | CSIRO/AAHL, University of Cambridge, Zoological Society (UK) | 71 |
SYBR qRT-PCR | NiV N gene | CSIRO/AAHL, University of Cambridge, Zoological Society (UK) | 71 |
Real-time RT-PCR | NiV/HeV P, M, N genes | CSIRO/AAHL, University of Cambridge, Zoological Society (UK) | 71 |
Multiplex bead-based real-time RT-PCR | NiV, HeV N and P genes | CSIRO/AAHL (Australia) | 89 |
Multiplex array card real-time RT-PCR | 26 multiplex panel including NiV N gene | CDC (Kenya, USA) | 90 |
Multiplex array card real-time RT-PCR | 21 multiplex panel including NiV N gene | Kenya Medical Research Institute (Kenya), CDC (Kenya, USA) | 91 |
*Adapted with permission from Springer Customer Service Centre GmBH: Springer Nature Indian Journal of Virology,66 copyright 2013; Springer Customer Service Centre GmBH: Springer Nature Springer eBook,67 copyright 2012; and World Organisation for Animal Health (OIE).70
AAHL, Australian Animal Health Laboratory; CDC, Centers for Disease Control and Prevention; CSIRO, Commonwealth Scientific and Industrial Research Organisation; HeV, Hendra virus; ICDDRB, International Centre for Diarrhoeal Disease Research, Bangladesh; IEDCR, Institute of Epidemiology, Disease Control and Research; NiV, Nipah virus; RT-PCR, reverse transcriptase PCR; qRT-PCR, quantitative RT-PCR.