Table 2

Antinuclear antigens on the diagnostic test strip

AntigenImmunoblot assay kit manufacture’s detail
nRNP/SmUridine 1-ribonuclear protein (U1-nRN) purified by affinity chromatography from calf and rabbit thymus
SmSmith uridine-1-5 ribonuclear protein antigen purified by affinity chromatography from bovine spleen and thymus. The Sm antigen contains the core proteins of snRNP particles. D protein is the main component of the Sm preparation
SS-ASoluble substance-A (60 kDa) purified by affinity chromatography from bovine spleen and thymus
Ro-52Recombinant Ro-52 (52 kDa). The corresponding human cDNA has been expressed with the baculovirus system in insect cells
SS-BSoluble substance-B antigen purified by affinity chromatography from calf and rabbit thymus
Scl-70DNA topoisomerase antigen purified by affinity chromatography from bovine and rabbit thymus
PM-SclPolymyositis–sclerosis overlap antigen. Recombinant PM-Scl100 whose corresponding human cDNA has been expressed with the baculovirus system in insect cells
Jo-1Cytoplasmic histidyl-tRNA synthetase antigen purified by affinity chromatography from calf and rabbit thymus
CENP-BRecombinant centromere protein B whose corresponding human cDNA has been expressed with the baculovirus system in insect cells
PCNAProliferating cell nuclear antigen. Recombinant PCNA (36 kDa) whose corresponding human cDNA has been expressed with the baculovirus system in insect cells
dsDNADouble-stranded DNA. The highly purified native, double-stranded DNA was isolated from salmon testes
NucleosomesNative nucleosomes purified from calf thymus
HistonesA mixture of individually purified histone types isolated from calf thymus
Rib. P-proteinRibosomal P proteins purified by affinity chromatography
AMA-M2Purified antimitochondrial M2 (pyruvate–dehydrogenase complex) antigen