RT Journal Article SR Electronic T1 PO 8418 NEW MALARIA EPIDEMIOLOGY IN COASTAL LAGOON OF BENIN: PLASMODIUM INFECTION IN ANOPHELES MELAS JF BMJ Global Health FD BMJ Publishing Group Ltd SP A35 OP A35 DO 10.1136/bmjgh-2019-EDC.90 VO 4 IS Suppl 3 A1 Claudiane Adigbonon A1 Benoît S Assogba A1 Luc S Djogbenou YR 2019 UL http://gh.bmj.com/content/4/Suppl_3/A35.1.abstract AB Introduction Malaria is a worldwide disease affecting many people particularly in the tropical and sub-tropical areas. It is caused by Plasmodium parasites and essentially transmitted by female mosquitoes belonging to the Anopheles genus. Our understanding of the infectivity of these vectors to Plasmodium is necessary to design sustainable strategies for their control. This aspect remains unknown in the coastal and lagoon area of Benin where Anopheles melas and Anopheles coluzzii are sympatric. This study aims to investigate the infectivity of these two vectors to Plasmodium to understand their role in malaria transmission in southern Benin.Methods Insecticide spray catch technique was used to collect females in 80 houses randomly selected in our study site. Three hundred and twenty (320) females were identified using PCR–species technique, Plasmodium infection was determined by the TaqMan method during the dry season. This assay detects all four malaria-causing species and discriminates Plasmodium falciparum from Plasmodium ovale, Plasmodium vivax and Plasmodium malariae (OVM).Results During the dry season, the sporozoïte rates were 0.2% and 0.3% for Anopheles melas and Anopheles coluzzii, respectively. However, we observed that positivity to the OVM (one of Plasmodium ovale, Plasmodium vivax and Plasmodium malariae species) was significantly higher in Anopheles melas (95%) than in Anopheles coluzzii (33.33%) (Chi-sq=15 857, df=1, p<0.001). These results indicated that Anopheles melas is more infected by one of the species Plasmodium ovale, Plamodium vivax and Plasmodium malariae than by Plasmodium falciparum, contrarily to Anopheles coluzzii.Conclusion These findings reinforce the debate on the role of Anopheles melas in malaria transmission in coastal lagoon areas of Benin.