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PA-101
FUNCTIONAL AND PHENOTYPIC CHARACTERISATION OF REGULATORY T (TREG) CELLS IN ANTIRETROVIRAL NAïVE HIV-1 INFECTED PEOPLE
  1. Georgia Ndzengue1,
  2. Ngu Loveline2,
  3. Carol Sake2,
  4. Abel Lissom2,
  5. Jules Tchadji2,
  6. Tchouangueu Flaurent2,
  7. Nadesh Nji1,
  8. Samuel Sosso1,
  9. Claudine Essomba2,
  10. François-Xavier Etoa2,
  11. Godwin Nchinda1
  1. 1CIRCB, Cameroon
  2. 2University of Yaoundé I, Cameroon

Abstract

Background Regulatory T cells (Tregs) function in dampening excessive immune activation in steady state. However during HIV-1 infection there is sustained immune activation and it is not known how Tregs function in this context. To optimise immunotherapeutic strategies based on Tregs for HIV-1 infected people we assessed the phenotypic and functional properties of these cells from antiretroviral naïve HIV-1 infected adults in Cameroon.

Methods Tregs were purified by magnetic sorting from PBMCs obtained from adults aged 21 to 65 years using microbeads according to the manufacturer's protocol (Miltenyi Biotec). The phenotypic properties of the purified Tregs were then determined by multiparametric flow cytometry. Tregs functions were assessed by measuring inflammatory cytokine formation by monocytes following co-culture with autologous Tregs in the presence of either polyICLC or CLO97. Samples were acquired on BD Fortessa X5 cytometer using BDFACS Diva Software and data analysed with FlowJo version 9.8.5. Graph Pad Prism 5 was used for statistical analysis.

Results Tregs were defined as CD4+CD25+CD127LoFoxP3+ cells. However, the strong correlation between Foxp3 with the combination of CD25+CD127Lo (r=0,965; p< 0,001, Pearson's correlation) allowed us to use these surface markers as previously reported for tracking Tregs in subsequent experiments. With respect to surface expression there was a significant elevation of HLA-DR /CD38 in Tregs from HIV-1-infected people when compared to HIV-participants. When purified Tregs were co-cultured with autologous monocytes in the presence polyICLC (a TLR 3 agonist) and CLO97 (TLR7/8 agonist) they escalated the intracellular formation of both TNF-α and IL-6 by monocytes. The escalation was significantly higher in co-cultures of cells from antiretroviral naïve HIV-1-infected people relative to seronegative participants.

Conclusions Dysregulation in Tregs function can exacerbate inflammatory cytokine formation.

This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/

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