Morpho-typing and molecular diversity of arbuscular mycorrhizal fungi in sub-tropical soils of Coimbatore region, Tamil Nadu, India

Indian J Microbiol. 2012 Jun;52(2):145-52. doi: 10.1007/s12088-011-0206-2. Epub 2011 Aug 13.

Abstract

The diversity potential of arbuscular mycorrhizal fungi (AMF) in three different tropical soils of southern part of India was assessed by traditional morpho-typing of AMF-spores and by culture-independent nested-PCR of internal transcribed spacer region of ribosomal genes. The population diversity of AMF in soil was strongly correlated with available P2O5 in soil. Among the three different soils, black-cotton soil had more diversified AMF species than alluvial and red sandy soils. Pooled data of morpho-typing and sequence-driven analysis revealed that Glomus, Gigaspora, Scutellospora and Acaulospora are the AMF genera present in these soils. The diversity of AMF in soil differs with the mycorrhiza colonizing the plant roots.

Keywords: Arbuscular mycorrhizal fungi; Diversity; Internal transcribed spacer; Morpho-typing; Nested-PCR.