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GENE VARIATION AND SUSPECTED PLASMODIUM FALCIPARUM HISTIDINE-RICH PROTEIN 2 GENE DELETION AND ITS IMPACT ON SENSITIVITY OF MALARIA RAPID DIAGNOSTIC TESTS IN SUDAN
  1. Muzamil Abdel Hamid1,
  2. Mona Awad-Elgeid1,
  3. Awad Nasr2
  1. 1IEND, University of Khartoum, Sudan
  2. 2University of Khartoum, Sudan

Abstract

Background Malaria rapid diagnostic tests (RDTs) play a significant role in malaria case management and case investigations. Variability or absence of antigens targeted by PfHRP2-based RDTs have been reported worldwide. However, little data is available concerning genetic variability within Sudanese Plasmodium falciparum isolates while variable sensitivity of PfHRP2 based RDTs has been observed. The objective was to find out the possible effect of PfHRP2 gene variation and suspected deletion on the performance of PfHRP2-based RDTs.

Methods Seventy-seven P. falciparum isolates were selected from three geographical regions of Sudan. Malaria HRP2-RDTs and Giemsa-stained blood films data were included for analysis. The pfhrp2 exon 2 fragments were amplified to study genetic variation and suspected deletion. Chi-square test was used for testing significance of results.

Results Forty percent (31/77) of P. falciparum isolates showed amplification for PfHRP2 (which revealed five alleles of different sizes), whereas 60% of isolates were PfHRP2 PCR-negative. There is a concordance of positive and negative rates on PfHRP2 RDT and gene amplification results of (35%) and (33%) respectively. Eighty-seven percent (78%) of RDTs positive isolates were PfHRP2 negative (p-value=0.001), while 4 out of 31 pfhrp2 positive isolates gave false negative results in RDT detection. Twenty out of 47 RDTs positive isolates were PfHRP2 negative (p-value=0.001). Plasmodium falciparum HRP2-RDTs showed higher sensitivity than microscopy in malaria detection (p-value=0.007).

Conclusions The study provided baseline data on genetic variation and suspected deletion in PfHRP2 and its potential effect on RDT performance.

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